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Purification Process of Recombinant Monoclonal Antibodies with Mixed Mode Chromatography by Sophie Maria; Gilles Joucla; Bertrand Garbay; Wilfrid Dieryck; Anne-Marie Lomenech; Xavier Santarelli; Charlotte Cabanne is a Chemistry article available to read on EtoBox.
What is Purification Process of Recombinant Monoclonal Antibodies with Mixed Mode Chromatography about?
An innovative process to purify mAb from CHO cell culture supernatant was developed. This three-step process involved two mixed mode resins and an anion exchange membrane. We used a human IgG mixture to determine the optimal conditions for each purification step. Thereafter, the whole process was evaluated and improved for the purification of a recombinant mAb produced in the supernatant of CHO cells. Once optimized, yield and purity of 88% and 99.9%, respectively were comparable to those obtained in a conventional process based on a capture step using protein A. In addition, aggregates, HCPs and DNA levels in the purified fraction were below regulatory specifications. Then we used mass spectrometry to identify contaminating proteins in the antibody fraction in order to highlight the behavior of HCPs.
Who reads Purification Process of Recombinant Monoclonal Antibodies with Mixed Mode Chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Sophie Maria; Gilles Joucla; Bertrand Garbay; Wilfrid Dieryck; Anne-Marie Lomenech; Xavier Santarelli; Charlotte Cabanne
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2015
- Language
- EN
- Field
- Chemistry (Physical Sciences)