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Enzymic analysis of NADPH metabolism in β-lactam-producing Penicillium chrysogenum: Presence of a mitochondrial NADPH dehydrogenase by Diana M. Harris; Jasper A. Diderich; Zita A. van der Krogt; Marijke A.H. Luttik; Léonie M. Raamsdonk; Roel A.L. Bovenberg; Walter M. van Gulik; Johannes P. van Dijken; Jack T. Pronk is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Enzymic analysis of NADPH metabolism in β-lactam-producing Penicillium chrysogenum: Presence of a mitochondrial NADPH dehydrogenase about?

Based on assumed reaction network structures, NADPH availability has been proposed to be a key constraint in beta-lactam production by Penicillium chrysogenum. In this study, NADPH metabolism was investigated in glucose-limited chemostat cultures of an industrial P. chrysogenum strain. Enzyme assays confirmed the NADP(+)-specificity of the dehydrogenases of the pentose-phosphate pathway and the presence of NADP(+)-dependent isocitrate dehydrogenase. Pyruvate decarboxylase/NADP(+)-linked acetaldehyde dehydrogenase and NADP(+)-linked glyceraldehyde-3-phosphate dehydrogenase were not detected. Although the NADPH requirement of penicillin-G-producing chemostat cultures was calculated to be 1.4-1.6-fold higher than that of non-producing cultures, in vitro measured activities of the major NADPH-providing enzymes were the same. Isolated mitochondria showed high rates of antimycin A-sensitive respiration of NADPH, thus indicating the presence of a mitochondrial NADPH dehydrogenase that oxidises cytosolic NADPH. The presence of this enzyme in P. chrysogenum might have important implications for stoichiometric modelling of central carbon metabolism and beta-lactam production and may provide

Who reads Enzymic analysis of NADPH metabolism in β-lactam-producing Penicillium chrysogenum: Presence of a mitochondrial NADPH dehydrogenase?

Author

Diana M. Harris; Jasper A. Diderich; Zita A. van der Krogt; Marijke A.H. Luttik; Léonie M. Raamsdonk; Roel A.L. Bovenberg; Walter M. van Gulik; Johannes P. van Dijken; Jack T. Pronk

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 1096-7176)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)