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ZmES genes encode peptides with structural homology to defensins and are specifically expressed in the female gametophyte of maize by Simone Cordts; Jörg Bantin; Peter E. Wittich; Erhard Kranz; Horst Lörz; T. Dresselhaus is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary All four members of a gene family, which are highly expressed in the cells of the female gametophyte (__ZmES1–4__: __Zea mays__ embryo sac), were isolated from a cDNA library of maize egg cells. High expression of __ZmES__ genes in the synergids around the micropylar region was detected in thin sections of maize ovaries. Single‐cell RT–PCR analyses with the various cells of the female gametophyte confirmed the expression in synergids and also showed expression in the egg cell and central cell, and low expression in the antipodals. The expression of the whole gene family is suppressed after fertilization of the embryo sac, and expression in two‐cell or later embryo stages or other tissues of maize could not be detected. In order to investigate __ZmES__ mRNA gradients in the highly polarized and vacuolized cells of the maize embryo sac, a whole‐mount __in situ__ protocol with isolated single cells was developed: as for total RNA, __ZmES__ transcripts are uniformly distributed in the cytoplasm of egg cell, synergids and central cell. __ZmES__ genes encode small, cysteine‐rich proteins with an N‐terminal signal peptide, probably for translocation into the embryo sac cell wal

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Author

Simone Cordts; Jörg Bantin; Peter E. Wittich; Erhard Kranz; Horst Lörz; T. Dresselhaus

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

2008

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)