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Modulation by neonicotinoids of honeybee α1/chicken Î22 hybrid nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes by Shigetou, Sho; Shimada, Shota; Makoto, Ihara; Matsuda, Kazuhiko is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Modulation by neonicotinoids of honeybee α1/chicken Î22 hybrid nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes about?

Neonicotinoids targeting insect nicotinic acetylcholine (ACh) receptors (insect nAChRs) are used for crop protection, but there is a concern about adverse effects on pollinators such as honeybees (Apis mellifera). Thus, we investigated the agonist actions of neonicotinoids (imidacloprid, thiacloprid and clothianidin) on A. mellifera α1 (Amα1)/chicken β2 hybrid nAChRs in Xenopus laevis oocytes according to the subunit stoichiometry of (Amα1)(β2) and (Amα1)(β2) using voltage-clamp electrophysiology. ACh activated (Amα1)(β2) and (Amα1)(β2) nAChRs with similar current amplitude. We investigated the agonist activity of imidacloprid, thiacloprid and clothianidin for the two hybrid nAChRs and found that: 1) imidacloprid showed higher affinity than clothianidin, whereas clothianidin showed higher efficacy than imidacloprid for the nAChRs; 2) Thiacloprid showed the highest agonist affinity and the lowest efficacy for the nAChRs. The Amα1/β2 subunit ratio influenced the efficacy of imidacloprid and thiacloprid, but hardly affected that of clothianidin. Hydrogen bond formation by the NH group in clothianidin with the main chain carbonyl of the loop B may account, at least in part, for the uni

Who reads Modulation by neonicotinoids of honeybee α1/chicken Î22 hybrid nicotinic acetylcholine receptors expressed in Xenopus laevis oocytes?

Author

Shigetou, Sho; Shimada, Shota; Makoto, Ihara; Matsuda, Kazuhiko

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0048-3575)

Published

2020

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)