Opening book details…
Can I read Glutamine Synthetase in Muscle Is Required for Glutamine Production during Fasting and Extrahepatic Ammonia Detoxification on EtoBox?
Glutamine Synthetase in Muscle Is Required for Glutamine Production during Fasting and Extrahepatic Ammonia Detoxification by Youji He; Theodorus B.M. Hakvoort; S. Eleonore Köhler; Jacqueline L.M. Vermeulen; D. Rudi de Waart; Chiel de Theije; Gabrie A.M. ten Have; Hans M.H. van Eijk; Cindy Kunne; Wilhelmina T. Labruyere; Sander M. Houten; Milka Sokolovic; Jan M. Ruijter; Nicolaas E.P. Deutz; Wouter H. Lamers is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Glutamine Synthetase in Muscle Is Required for Glutamine Production during Fasting and Extrahepatic Ammonia Detoxification about?
The main endogenous source of glutamine is de novo synthesis in striated muscle via the enzyme glutamine synthetase (GS). The mice in which GS is selectively but completely eliminated from striated muscle with the Cre-loxP strategy (GS-KO/M mice) are, nevertheless, healthy and fertile. Compared with controls, the circulating concentration and net production of glutamine across the hindquarter were not different in fed GS-KO/M mice. Only a approximately 3-fold higher escape of ammonia revealed the absence of GS in muscle. However, after 20 h of fasting, GS-KO/M mice were not able to mount the approximately 4-fold increase in glutamine production across the hindquarter that was observed in control mice. Instead, muscle ammonia production was approximately 5-fold higher than in control mice. The fasting-induced metabolic changes were transient and had returned to fed levels at 36 h of fasting. Glucose consumption and lactate and ketone-body production were similar in GS-KO/M and control mice. Challenging GS-KO/M and control mice with intravenous ammonia in stepwise increments revealed that normal muscle can detoxify approximately 2.5 micromol ammonia/g muscle.h in a muscle GS-dependen
Who reads Glutamine Synthetase in Muscle Is Required for Glutamine Production during Fasting and Extrahepatic Ammonia Detoxification?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Youji He; Theodorus B.M. Hakvoort; S. Eleonore Köhler; Jacqueline L.M. Vermeulen; D. Rudi de Waart; Chiel de Theije; Gabrie A.M. ten Have; Hans M.H. van Eijk; Cindy Kunne; Wilhelmina T. Labruyere; Sander M. Houten; Milka Sokolovic; Jan M. Ruijter; Nicolaas E.P. Deutz; Wouter H. Lamers
- Publisher
- Elsevier BV
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)