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Two types of Na ^+^‐currents in cultured rat optic nerve astrocytes: Changes with time in culture and with age of culture derivation by Ph.D. H. Sontheimer; J. E. Minturn; J. A. Black; B. R. Ransom; S. G. Waxman is a Neuroscience article available to read on EtoBox.

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## Abstract Na^+^ channel expression was studied in cultures of rat optic nerve astrocytes using whole‐cell voltage‐clamp recordings. Astrocytes from postnatal day 7 rat optic nerve (RON) expressed two distinct types of Na^+^ currents, which had significantly different __h__~∞~ curves. Stellate, GFAP ^+^/A2B5 ^+^ astrocytes showed currents with h~∞~ curve midpoints close to−65 mV, similar to Na ^+^ currents in most neurons. In contrast, flat fibroblast‐like GFAP ^+^/A2B5^−^ astrocytes showed Na^+^ currents with __h__~∞~ midpoints around −85 mV, almost 20 mV more hyperpolarized than in neurons or A2B5 ^+^ astrocytes. Interestingly, Na ^+^ current expression was maintained in A2B5 Na^+^ astrocytes but began to decrease in A2B5^−^ astrocytes after 6 days in vitro (DIV) and fell to or below the level of detection (i.e., 1 pA/pF) at 12 DIV. Astrocytes cultured from neonatal rats (P0) are almost exclusively GFAP ^+^/A2B5‐. These cells did not display measurable Na ^+^ currents when studied at 2 DIV; however, Na^+^ current was observed after 5 DIV in A2B5^−^ astrocytes from these neonatal (P0) cultures. These findings were substantiated by immunocytochemical experiments using 7493, an ant

Who reads Two types of Na ^+^‐currents in cultured rat optic nerve astrocytes: Changes with time in culture and with age of culture derivation?

Author

Ph.D. H. Sontheimer; J. E. Minturn; J. A. Black; B. R. Ransom; S. G. Waxman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1991

Language

EN

Field

Neuroscience (Life Sciences)