Can I read Generation of Cardiomyocytes from Human-induced Pluripotent Stem Cells Resembling Atrial Cells with Ability to Respond to Adrenoceptor Agonists on EtoBox?

Generation of Cardiomyocytes from Human-induced Pluripotent Stem Cells Resembling Atrial Cells with Ability to Respond to Adrenoceptor Agonists by Faizzan S. Ahmad; Yongcheng Jin; Alexander Grassam-Rowe; Yafei Zhou; Meng Yuan; Xuehui Fan; Rui Zhou; Razik Mu-u-min; Christopher O'Shea; Ayman M. Ibrahim; Wajiha Hyder; Yasmine Aguib; Magdi Yacoub; Davor Pavlovic; Yanmin Zhang; Xiaoqiu Tan; Ming Lei; Derek A. Terrar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Generation of Cardiomyocytes from Human-induced Pluripotent Stem Cells Resembling Atrial Cells with Ability to Respond to Adrenoceptor Agonists about?

Atrial fibrillation (AF) is the most common chronic arrhythmia presenting a heavy disease burden. We report a new approach for generating cardiomyocytes (CMs) resembling atrial cells from human-induced pluripotent stem cells (hiPSCs) using a combination of Gremlin 2 and retinoic acid treatment. More than 40% of myocytes showed rod-shaped morphology, expression of CM proteins (including ryanodine receptor 2, __α__-actinin-2 and F-actin) and striated appearance, all of which were broadly similar to the characteristics of adult atrial myocytes (AMs). Isolated myocytes were electrically quiescent until stimulated to fire action potentials with an AM profile and an amplitude of approximately 100 mV, arising from a resting potential of approximately −70 mV. Single-cell RNA sequence analysis showed a high level of expression of several atrial-specific transcripts including __NPPA__, __MYL7__, __HOXA3__, __SLN__, __KCNJ4__, __KCNJ5__ and __KCNA5__. Amplitudes of calcium transients recorded from spontaneously beating cultures were increased by the stimulation of __α__-adrenoceptors (activated by phenylephrine and blocked by prazosin) or __β__-adrenoceptors (activated by isoproterenol and bl

Who reads Generation of Cardiomyocytes from Human-induced Pluripotent Stem Cells Resembling Atrial Cells with Ability to Respond to Adrenoceptor Agonists?

Author

Faizzan S. Ahmad; Yongcheng Jin; Alexander Grassam-Rowe; Yafei Zhou; Meng Yuan; Xuehui Fan; Rui Zhou; Razik Mu-u-min; Christopher O'Shea; Ayman M. Ibrahim; Wajiha Hyder; Yasmine Aguib; Magdi Yacoub; Davor Pavlovic; Yanmin Zhang; Xiaoqiu Tan; Ming Lei; Derek A. Terrar

Publisher

The Royal Society

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)