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Npa7: A Novel Designed Bispecific Peptide That Protects Human Cardiomyocytes Against Oxidative Stress Through Gc-a/mas Receptors by Xiaoyu Ma; Xiao Ma; Jeson Sangaralingham; John C. Burnett is a Medicine article available to read on EtoBox.

What is Npa7: A Novel Designed Bispecific Peptide That Protects Human Cardiomyocytes Against Oxidative Stress Through Gc-a/mas Receptors about?

Background: Cardiovascular diseases (CVD), such as hypertension and heart failure, is a leading cause of mortality and morbidity. Excess cardiomyocyte oxidative stress is a major metabolic abnormality in CVD. Thus therapies that reduce oxidative stress in the heart may lead to a reduction in CVD related adverse outcomes. Recently, we bioengineered a bispecific peptide NPA7 that simultaneously co-activates the protective GC-A/cGMP and MasR/cAMP pathways. Our previous research showed that NPA7 possesses cardiorenal protective properties. However, the antioxidant effects of NPA7 within the heart is unknown. Hence the objective of this study was to determine if the GC-A and MasR systems, via NPA7, reduces oxidative stress in human cardiomyocytes (HCMs). Methods: Primary HCMs were pretreated with vehicle or NPA7 (10 uM) followed by 400 uM hydrogen peroxide (H 2 O 2 ) to induce oxidative stress. Intracellular ROS was measured by dihydroethidium (DHE) staining and by determining the mRNA and protein levels of NADPH oxidase (NOX) via real-time PCR and western blot. The cell viability was evaluated by MTT assay. HCMs were transfected with the siRNA target GC-A and MasR for 24 h and then tre

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Author

Xiaoyu Ma; Xiao Ma; Jeson Sangaralingham; John C. Burnett

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Medicine (Health Sciences)