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Glial Restricted Precursors Protect Against Chronic Glutamate Neurotoxicity of Motor Neurons in Vitro by Nicholas J. Maragakis; Mahendra S. Rao; Jeronia Llado; Victor Wong; Haipeng Xue; Andrea Pardo; Joseph Herring; Douglas Kerr; Carol Coccia; Jeffrey D. Rothstein is a Neuroscience article available to read on EtoBox.

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## Abstract We have examined the expression of glutamate transporters in primary and immortalized glial precursors (GRIPs). We subsequently transduced these cells with the GLT1 glutamate transporter and examined the ability of these cells to protect motor neurons in an organotypic spinal cord culture. We show that glial restricted precursors and GRIP‐derived astrocytes predominantly express glutamate transporters GLAST and GLT1. Oligodendrocyte differentiation of GRIPs results in downregulation of all glutamate transporter subtypes. Having identified these precursor cells as potential vectors for delivering glutamate transporters to regions of interest, we engineered a line of GRIPS that overexpress the glutamate transporter GLT1. These cells (G3 cells) have a nearly fourfold increase in glutamate transporter expression and at least a twofold increase in the V~max~ for glutamate transport. To assess whether G3 seeding can protect motor neurons from chronic glutamate neurotoxicity, G3s were seeded onto rat organotypic spinal cord cultures. These cultures have previously been used extensively to understand pathways involved in chronic glutamate neurotoxicity of motor neurons. After G

Who reads Glial Restricted Precursors Protect Against Chronic Glutamate Neurotoxicity of Motor Neurons in Vitro?

Author

Nicholas J. Maragakis; Mahendra S. Rao; Jeronia Llado; Victor Wong; Haipeng Xue; Andrea Pardo; Joseph Herring; Douglas Kerr; Carol Coccia; Jeffrey D. Rothstein

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2005

Language

EN

Field

Neuroscience (Life Sciences)