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The document presents a rapid and simple isocratic HPLC method for determining neurotransmitters (DOPAC, norepinephrine, dopamine, and serotonin) in mouse brain homogenate using direct fluorescence detection. The method, which separates the analytes in 15 minutes without the need for sample pre-purification or derivatization, has been optimized and validated, showing high recovery rates and low relative standard deviations. It is demonstrated to be effective in analyzing samples from a mouse model of Parkin

Author

Carlos Almeida

Language

EN