About this Biochemistry, Genetics and Molecular Biology article

Computer-controlled Pulse Modulation System for Analysis of Photoacoustic Signals in the Time Domain by Jrg Kolbowski; Heinz Reising; Ulrich Schreiber is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

A newly developed photoacoustic system for measurement of photosynthetic reactions in intact leaves is described. The system is based on pulsed light-emitting diodes, the pulse program and pulse response analysis being computer controlled. Separation of various components in the overall photoacoustic signal is achieved by curve fitting analysis of the responses following individual measuring light pulses in the millisecond time domain. This procedure is in distinction to the conventionally used analysis in the frequency domain, with the advantage that various signal components are obtained by on-line deconvolution, yielding simultaneous recordings of photothermal (complement of energy storage) and photobaric (evolution and uptake) signals. The basic components of the new system are described by block diagrams and the principal steps for deconvolution of the overall photoacoustic response are outlined. An example of application with simultaneous recording of chlorophyll fluorescence is given. It is apparent that the photobaric uptake component represents a significant part of the overall signal, particularly during induction of photosynthesis after dark-adaptation. This component pr

Author

Jrg Kolbowski; Heinz Reising; Ulrich Schreiber

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0166-8595)

Published

1990

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)