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Analysis of the κ Light Chain Variable Region in Multiple Myeloma by Christos Kosmas; Nora‐Athina Viniou; Kostas Stamato poulos; Nigel S. Courtenay‐Luck; Theodora Papadaki; Panagoula Kollia; George Paterakis; Dimitra Anagnostou; Xenophon Yataganas; Dimitris Loukopoulos is a Medicine article available to read on EtoBox.
What is Analysis of the κ Light Chain Variable Region in Multiple Myeloma about?
The study of immunoglobulin heavy chain gene rearrangements in multiple myeloma has revealed extensive divergence from the germline sequences, but no intraclonal diversity with disease evolution. Our study investigated the state of the rearranged κ light chain variable region (Vκ) gene segments, as well as abortive Vκ family gene usage in cases of multiple myeloma expressing λ light chain. We studied 11 cases of κ and five cases of λ light chain‐expressing multiple myeloma. Total cellular RNA was extracted from the bone marrow of patients with overt disease and subjected to reverse transcription‐polymerase chain reaction (RT‐PCR) analysis to amplify clonally rearranged variable region sequences. Direct nucleotide sequencing by the dideoxy‐chain termination method was performed on the RT‐PCR products. We did not observe preferential usage of certain Vκ gene families. Mutation frequencies of the Vκ segments varied in number. In the majority of cases, extensive somatic mutations occurred within the complementarity determining regions (CDRs) of Vκ, whereas only a limited degree of divergence from the germline was observed in others. In all cases studied, replacement mutations tended to
Who reads Analysis of the κ Light Chain Variable Region in Multiple Myeloma?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Christos Kosmas; Nora‐Athina Viniou; Kostas Stamato poulos; Nigel S. Courtenay‐Luck; Theodora Papadaki; Panagoula Kollia; George Paterakis; Dimitra Anagnostou; Xenophon Yataganas; Dimitris Loukopoulos
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)
- Published
- 1996
- Language
- EN
- Field
- Medicine (Health Sciences)