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Mitochondrial activities in human cultured skin fibroblasts contaminated byMycoplasma hyorhinis by Niklas Darin; Norman Kadhom; Jean-Jacques Brière; Dominique Chretien; Cécile M Bébéar; Agnès Rötig; Arnold Munnich; Pierre Rustin is a scholarly article available to read on EtoBox.

What is Mitochondrial activities in human cultured skin fibroblasts contaminated byMycoplasma hyorhinis about?

Background: Mycoplasma contaminations are a recurrent problem in the use of cultured cells, including human cells, especially as it has been shown to impede cell cycle, triggering cell death under various conditions. More specific consequences on cell metabolism are poorly known. Results: Here we report the lack of significant consequence of a heavy contamination by the frequently encountered mycoplasma strain, M. hyorhinis, on the determination of respiratory chain activities, but the potential interference when assaying citrate synthase. Contamination by M. hyorhinis was detected by fluorescent imaging and further quantified by the determination of the mycoplasma-specific phosphate acetyltransferase activity. Noticeably, this latter activity was not found equally distributed in various mycoplasma types, being exceptionally high in M. hyorhinis. ## Conclusion: While we observed a trend for respiration reduction in heavily contaminated cells, no significant and specific targeting of any respiratory chain components could be identified. This suggested a potential interference with cell metabolism rather than direct interaction with respiratory chain components.

Author

Niklas Darin; Norman Kadhom; Jean-Jacques Brière; Dominique Chretien; Cécile M Bébéar; Agnès Rötig; Arnold Munnich; Pierre Rustin

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central; Springer Science and Business Media LLC (ISSN 1471-2091)

Published

2003

Language

EN