Can I read Induction of human B2 bradykinin receptor mRNA and membrane receptors by IFNγ on EtoBox?

Induction of human B2 bradykinin receptor mRNA and membrane receptors by IFNγ by Chien-Cheng Lung; Mark A. Jagels; Pamela J. Daffern; Eng M. Tan; Bruce L. Zuraw is a Immunology and Microbiology article available to read on EtoBox.

What is Induction of human B2 bradykinin receptor mRNA and membrane receptors by IFNγ about?

A potential mechanism for the increased sensitivity of inflamed tissues to bradykinin is the upregulation of bradykinin receptor expression. We report that recombinant human IFNg stimulated a concentration-dependent increase in cell surface bradykinin receptor expression in intact T24 human epithelial-like cells, determined by radioligand binding analysis. w 3 x Analysis of specific H -bradykinin binding revealed that IFNg-treated cells had a two-to threefold increase in bradykinin receptor number compared to the controls with no effect on receptor affinity. The ability of IFNg to stimulate increased bradykinin receptor expression was abrogated by treatment with either the transcription inhibitor actinomycin D or the protein synthesis inhibitor cycloheximide. IFNg enhanced steady-state human B2 bradykinin receptor mRNA expression in the T24 cells in a dose-dependent manner. B2 bradykinin receptor mRNA expression was increased as early as 1 h following IFNg stimulation, and continued to accumulate for 24 h. Bradykinin-stimulated intracellular calcium mobilization was also increased in IFNg-treated T24 cells compared to controls. The ability of IFNg to upregulate B2 bradykinin recept

Who reads Induction of human B2 bradykinin receptor mRNA and membrane receptors by IFNγ?

Author

Chien-Cheng Lung; Mark A. Jagels; Pamela J. Daffern; Eng M. Tan; Bruce L. Zuraw

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0162-3109)

Published

1998

Language

EN

Field

Immunology and Microbiology (Life Sciences)