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Uptake of Isolated Plant Chromosomes by Plant Protoplasts by L. Szabados; Gy. Hadlaczky; D. Dudits is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Uptake of Isolated Plant Chromosomes by Plant Protoplasts about?
For mass isolation of plant metaphase chromosomes, cultured cells of wheat (Triticurn m o n o c o c - cum) and parsley ( P e t r o s e l i n u m hortense) were synchronized by hydroxyurea and colchicine treatment. This synchronization procedure resulted in high mitotic synchrony, especially in suspension cultures of parsley in which 80% of the cells were found to be at the metaphase stage. Mitotic protoplasts isolated from these synchronized cell cultures served as a source for isolation of chromosomes. The described isolation and purification method yielded relatively pure chromosome suspension. The uptake of the isolated plant chromosomes into recipient wheat, parsley, and maize protoplasts was induced by polyethylene-glycol treatment. Cytological studies provided evidences for uptake of plant chromosomes into plant protoplasts.
Who reads Uptake of Isolated Plant Chromosomes by Plant Protoplasts?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- L. Szabados; Gy. Hadlaczky; D. Dudits
- Publisher
- Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)
- Published
- 1981
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)