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Is Radiation Damage Dependent on the Dose Rate Used During Macromolecular Crystallography Data Collection? by Hanna-Kirsti S. Leiros; Joanna Timmins; Raimond B. G. Ravelli; Seán M. McSweeney is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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This paper focuses on the radiation-damage effects when applying the same total X-ray dose to protein crystals at different dose rates. These experiments have been performed on both a selenomethionated protein and on bovine trypsin using dose rates that span nearly two orders of magnitude. The results show no clear dose-rate effect on the global indicators of radiation damage, but a small measurable dose-rate effect could be found when studying specific radiation damage. It is hypothesized that this observed dose-rate effect relates to differences in the steady-state free-radical concentration.

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Author

Hanna-Kirsti S. Leiros; Joanna Timmins; Raimond B. G. Ravelli; Seán M. McSweeney

Publisher

International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)