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Human BMP‐7/OP‐1 induces the growth and differentiation of adipocytes and osteoblasts in bone marrow stromal cell cultures\* by Theresa L. Chen; Wen‐Jun Shen; Fredric B. Kraemer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract We studied the effects of BMP‐7/OP‐1 on growth and differentiation of bone marrow stromal cells. BMS2, a mouse bone marrow stromal cell line capable of differentiating into adipocytes and osteoblasts, were treated in a serum‐free medium containing differentiation agents that favor the expression of both lineages. BMP‐7/ OP‐1 stimulated cell proliferation and differentiation concomitantly. These effects were dose‐ and growth phase‐dependent. Cells were more sensitive to the treatment early in the culture (30–40% confluence) with a significant increase in cell proliferation and markers of differentiation at low concentrations. When treated later in the growth phase (90–100% confluence), no significant increase in cell proliferation was seen. The concentration requirement for cells later in the culture to reach an equivalent degree of differentiation was 3–10‐ fold higher than for cells treated early. In both cases, the effects on adipocyte differentiation were biphasic; low concentrations stimulated adipocyte differentiation which was inhibited at higher concentrations where stimulation of osteoblast markers were observed. We conclude that cell proliferation and cell diff

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Author

Theresa L. Chen; Wen‐Jun Shen; Fredric B. Kraemer

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)