Can I read Adsorption immobilization of Escherichia coli phytase on probiotic Bacillus polyfermenticus spores on EtoBox?

Adsorption immobilization of Escherichia coli phytase on probiotic Bacillus polyfermenticus spores by Eun-Ah Cho; Eui-Joong Kim; Jae-Gu Pan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Adsorption immobilization of Escherichia coli phytase on probiotic Bacillus polyfermenticus spores about?

The immobilization of enzymes on edible matrix supports is of great importance for developing stabilized feed enzymes. In this study, probiotic Bacillus spores were explored as a matrix for immobilizing Escherichia coli phytase, a feed enzyme releasing phosphate from phytate. Because Bacillus spore is inherently resistant to heat, solvents and drying, they were expected to be a unique matrix for enzyme immobilization. When mixed with food-grade Bacillus polyfermenticus spores, phytases were adsorbed to their surface and became immobilized. The amount of phytase attached was 28.2 ± 0.7 mg/g spores, corresponding to a calculated activity of 63,960 U/g spores; however, the measured activity was 41,120 ± 990.1 U/g spores, reflecting a loss of activity upon adsorption. Immobilization increased the half life (t 1/2 ) of the enzyme three-to ten-fold at different temperatures ranging from 60 to 90 • C. Phytase was bound to the spore surface to the extent that ultrasonication treatment was not able to detach phytases from spores. Desorption of spore-immobilized phytase was only achieved by treatment with 1 M NaCl, 10% formic acid in 45% acetonitrile, SDS, or urea, suggesting that adsorption

Who reads Adsorption immobilization of Escherichia coli phytase on probiotic Bacillus polyfermenticus spores?

Author

Eun-Ah Cho; Eui-Joong Kim; Jae-Gu Pan

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)