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Determination of cells' water membrane permeability: Unexpected high osmotic permeability of Saccharomyces cerevisiae by I. Martínez de Marañón; P. Gervais; P. Molin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Determination of cells' water membrane permeability: Unexpected high osmotic permeability of Saccharomyces cerevisiae about?

Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time constant has been proposed allowing an accurate Lp estimation even though the mixing time constant is higher than the cell time constant obtained in response to a perfect shock. The estimated Lp values of human leukemia K562 cells were found to be the same whatever the mixing time constant. The Lp value of Saccharomyces cerevisiae could not be exactly estimated. However, S. cerevisiae has unexpectedly high water permeability, im- plying that this yeast may contain water channels in the membrane.

Who reads Determination of cells' water membrane permeability: Unexpected high osmotic permeability of Saccharomyces cerevisiae?

Author

I. Martínez de Marañón; P. Gervais; P. Molin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)