Can I read Role for TGF-β1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages on EtoBox?

Role for TGF-β1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages by Lara T. Diemel; Samuel J. Jackson; M. Louise Cuzner is a Neuroscience article available to read on EtoBox.

What is Role for TGF-β1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages about?

## Abstract The increase in myelin basic protein (MBP) synthesis observed in brain aggregate cultures supplemented with macrophages is reflected in elevated supernatant protein levels of the key promoters of oligodendrocyte proliferation, fibroblast growth factor‐2 (FGF‐2) and platelet‐derived growth factor‐AA (PDGF‐AA), during the premyelinating phase. Although supernatant levels of transforming growth factor‐β1 (TGF‐β1), the most abundant growth factor produced at the transcriptional and translational levels by phagocytic macrophages, were reduced at this stage, it was the only growth factor for which mRNA expression was increased significantly in macrophage‐enriched cultures. TGF‐β1, which supports oligodendrocyte differentiation, was increased in the supernatant of macrophage‐enriched cultures only after the onset of myelinogenesis. Hence, standard cultures treated with TGF‐β1 during the premyelinating period reproduced effects of macrophage supplementation, inducing an increase in MBP synthesis and in PDGF‐AA and FGF‐2 bioavailability. A similar increase in MBP synthesis in PDGF‐AA treated cultures emphasises its central role in oligodendrocyte progenitor proliferation. In con

Who reads Role for TGF-β1, FGF-2 and PDGF-AA in a myelination of CNS aggregate cultures enriched with macrophages?

Author

Lara T. Diemel; Samuel J. Jackson; M. Louise Cuzner

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2003

Language

EN

Field

Neuroscience (Life Sciences)