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Mucin Production by Colon Cancer Cells Cultured in Serum‐free Medium by Francisco X. Real; Gustavo Egea; Clara Francí; Martina H. Schüssler; Mai Xu; Sydney Welt is a Medicine article available to read on EtoBox.

What is Mucin Production by Colon Cancer Cells Cultured in Serum‐free Medium about?

Although many colon cancer cell lines are available for study, few of them exhibit differentiated properties. When cultured in medium containing fetal bovine serum, WiDr cells (WiDr-FBS) show an undifferentiated phenotype: growth as a multilayer of cells adherent to plastic and lack of polarization, brush border, and much vacuoles. In contrast, WiDr cells cultured in a chemically-defined serum-free medium containing insulin, transferrin and selenium (WiDr-ITS) grow as clusters of nonadherent cells with abundant desmosomes and tight junctions, microvilli and electron-lucid vacuoles. As WiDr-FBS cells, WiDr-ITS are not polarized. WiDr-ITS cells show a marked enhancement in mucin synthesis as demonstrated by: periodic acid-Schiff and Alcian blue stains, electron microscopy, immunohistochemistry using monoclonal antibodies (MAbs) reactive with mucin-associated epitopes, immune electron microscopy and immunochemical analysis using Western blots. In comparison with WiDr-FBS cells, WiDr-ITS cells showed strong expression of Tn, sialyl-Tn, blood group A and CEA. When mouse MAbs were used, higher levels of the MUC I gene product were detected in WiDr-ITS than in WiDr-FBS cells. The full spe

Who reads Mucin Production by Colon Cancer Cells Cultured in Serum‐free Medium?

Author

Francisco X. Real; Gustavo Egea; Clara Francí; Martina H. Schüssler; Mai Xu; Sydney Welt

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1991

Language

EN

Field

Medicine (Health Sciences)