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Brain Region Specificity in Reactive Oxygen Species Production and Maintenance of Redox Balance by Andrey Y. Vinokurov; Olga A. Stelmashuk; Polina A. Ukolova; Evgeny A. Zherebtsov; Andrey Y. Abramov is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Brain Region Specificity in Reactive Oxygen Species Production and Maintenance of Redox Balance about?

The brain produces various reactive oxygen species in enzymatic and non-enzymatic reactions as a by-product of metabolism and/or for redox signaling. Effective antioxidant system in the brain cells maintains redox balance. However, neurons and glia from some brain regions are more vulnerable to oxidative stress in ischemia/reperfusion, epilepsy, and neurodegenerative disorders than the rest of the brain. Using fluorescent indicators in live cell imaging and confocal microscopy, we have measured the rate of cytosolic and mitochondrial reactive oxygen species production, lipid peroxidation, and glutathione levels in cortex, hippocampus, midbrain, brain stem and cerebellum in acute slices of rat brain. We have found that the basal rate of ROS production is at its highest in brain stem and cerebellum, and that it is mainly generated by glial cells. Activation of neurons and glia by glutamate and ATP led to maximal rates of ROS production in the midbrain compared to the rest of the brain. Mitochondrial ROS had only minor implication to the total ROS production with maximal values in the cortex and minimal in the midbrain. The basal rate of lipid peroxidation was higher in the midbrain a

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Author

Andrey Y. Vinokurov; Olga A. Stelmashuk; Polina A. Ukolova; Evgeny A. Zherebtsov; Andrey Y. Abramov

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)