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Application of Two-Dimensional Total Correlation Spectroscopy for Structure Determination of Individual Inositol Phosphates in a Mixture by Kermit Johnson; Laura G. Barrientos; Long Le; Pushpalatha P.N. Murthy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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and references therein). Establishing the specificity of The discovery of the second messenger role of myoinositol phosphate-metabolizing enzymes has always inositol(1,4,5)trisphosphate has triggered tremenbeen a challenge because of the need to separate and dous interest in investigating the structures and meidentify the structures of individual inositol phostabolism of inositol phosphates. The structures of phates in a mixture containing stereo-and regioisothese compounds are established by first purifying mers. This problem is particularly acute when investithe compounds of interest and then identifying the gating the specificity of phytases, a group of enzymes structures through chemical degradation or NMR that hydrolyze phytic acid to a series of lower phosanalysis. In this paper we describe a method that phoric acid esters of myo-inositol and, in some cases, allows simultaneous determination, without separafinally to myo-inositol itself (9-11). tion, of the structures of multiple inositol phos- ## Structures of inositol phosphates have been estab- phates in a mixture. The use of two-dimensional total lished by first purifying the compound, which often recorrelation spectrosco

Who reads Application of Two-Dimensional Total Correlation Spectroscopy for Structure Determination of Individual Inositol Phosphates in a Mixture?

Author

Kermit Johnson; Laura G. Barrientos; Long Le; Pushpalatha P.N. Murthy

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)