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Gene Expression Profiles of Human Promyelocytic Leukemia Cell Lines Exposed to Volatile Organic Compounds by Sailendra Nath Sarma; Youn-Jung Kim; Jae-Chun Ryu is a Environmental Science article available to read on EtoBox.

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Benzene, toluene, o-xylene, ethylbenzene, trichloroethylene and dichloromethane are the most widely used volatile organic compounds (VOCs), and their toxic mechanisms are still undefined. This study analyzed the genome-wide expression profiles of human promyelocytic leukemia HL-60 cells exposed to VOCs using a 35-K whole human genome oligonucleotide microarray to ascertain potential biomarkers. Genes with a significantly increased expression levels (over 1.5-fold and p-values <0.05) with six VOCs were then classified with gene ontology and KEGG pathway annotation. At IC 20 doses identified genes were functionally categorized as being involved in cytokine-cytokine receptor interactions and the tolllike receptor signaling pathway, whereas exposure at IC 50 doses identified genes associated with the p53 signaling pathway, apoptosis, and natural killer cell-mediated cytotoxicity pathway. Functionally important immune response-and apoptosis-related genes were further validated by real-time RT-PCR. The results showed that IFIT1, IFIT2, IFIT3, USP18, INFGR2, PMAIP1, GADD45A, NFKBIA, TNFAIP3, and BIRC3 genes altered their expression profiles in a dose-dependent manner. Similar expressions

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Author

Sailendra Nath Sarma; Youn-Jung Kim; Jae-Chun Ryu

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-483X)

Published

2010

Language

EN

Field

Environmental Science (Life Sciences)