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Diimidoesters: Role in electron and light microscopy by Margaret Tzaphlidou; Demetrios P. Matthopoulos is a Materials Science article available to read on EtoBox.

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Diimidoesters are a class of bifunctional reagents which cross-link by reacting with a-and v-amino groups of proteins. The structure and properties ofthese reagents as well as their reaction with proteins or cells are described. The importance ofthe type and concentration ofthe buffer and ofthe pH as well as the osmolarity and penetration of these fixatives are discussed. Possible artifacts produced during fixation and the use of diimidoesters in enzyme cytochemistry and immunocytochemistry are also reported. A comparison ofthe crosslinking and structures obtained by dumidoesters with those obtained by aldehydes is stated.

Who reads Diimidoesters: Role in electron and light microscopy?

Author

Margaret Tzaphlidou; Demetrios P. Matthopoulos

Publisher

Elsevier Science; Elsevier ; Pergamon Press Ltd.; Elsevier BV (ISSN 0739-6260)

Published

1988

Language

EN

Field

Materials Science (Physical Sciences)