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Upregulation of the long non-coding RNA SPRY4-IT1 indicates a poor prognosis and promotes tumorigenesis in ovarian cancer by Li, Hongxia; Liu, Chunhua; Lu, Zhanbin; Chen, Li; Wang, Juan; Li, Yindi; Ma, Huiping is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Biomedicine et Pharmacotherapy, 88 (2017) 529-534. doi:10.1016/j.biopha.2017.01.037 Upregulation of the long non-coding RNA SPRY4-IT1 indicates a poor prognosis and promotes tumorigenesis in ovarian cancer 1 1 Introduction 1 2 Materials and methods 2 2.1 Patients and tissue samples 2 2.2 Cell lines and culture conditions 2 2.3 RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) 2 2.4 Small interfering RNA transfection 2 2.5 Cell proliferation assay 2 2.6 Clonogenic assay 3 2.7 Flow cytometric analysis 3 2.8 Statistical analysis 3 3 Results 3 3.1 Expression of SPRY4-IT1 was up-regulated in OC tissues 3 3.2 Relationship of SPRY4-IT1 expression level with the clinicopathological factors in OC patients 3 3.3 High expression of SPRY4-IT1 predicts poor prognosis in OC patients 3 3.4 Diagnostic value of SPRY4-IT1 4 3.5 Knockdown of SPRY4-IT1 inhibits growth of OVCAR3 and SKOV3 cells in vitro 4 4 Discussion 5 5 Conclusion 6 Conflicts of interest 6 Acknowledgments 6 References 6 Long non-coding RNA,SPRY4-IT1,Ovarian cancer,Prognosis

Who reads Upregulation of the long non-coding RNA SPRY4-IT1 indicates a poor prognosis and promotes tumorigenesis in ovarian cancer?

Author

Li, Hongxia; Liu, Chunhua; Lu, Zhanbin; Chen, Li; Wang, Juan; Li, Yindi; Ma, Huiping

Publisher

Elsevier Science; Elsevier ; Elsevier Masson; Elsevier BV (ISSN 0753-3322)

Published

2017

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)