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What is FSC-A vs. FSC-H Scaling Explained about?

The document discusses why the FSC-A and FSC-H values for cells measured by flow cytometry are not always equal, and how to adjust the FSC-A scaling parameter to make them equal. Specifically, it notes that the FSC-H value is based on pulse height, while FSC-A captures the full pulse area, so FSC-A will always be greater than or equal to FSC-H for cells larger than the laser beam width. It recommends adjusting the FSC-A scaling parameter to place cells on a 45 degree angle on a FSC-A vs FSC-H plot to make t

Author

brian8576

Language

EN