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Jianpi Jiedu Recipe inhibits colorectal cancer liver metastasis via regulating ITGBL1-rich extracellular vesicles mediated ... 1 Introduction 2 Materials and methods 2 Cell culture 2 EVs extraction by ultracentrifugation 2 EVs characterization by electron microscopy and LM10 nanoparticle characterization system 2 Western blot 2 Plasmid construction and cell transfection 2 Real time PCR 3 Colony formation assay 3 Transwell assay 3 Wound healing assay 3 Preparation of alcohol extract of JPJDR 3 In vivo analysis 3 HE and immunohistological staining analysis 3 Flow cytometric sorting 3 Tissue immunofluorescence 3 Statistical analysis 4 Results 4 JPJDR reduced the ITGBL1 levels in CRC cells-derived EVs 4 JPJDR inhibited CRC metastasis and growth via regulating ITGBL1-contained EVs pretreated fibroblasts 4 JPJDR decreased fibroblasts activation by regulating ITGBL1-rich EVs mediated TNFAIP3-NF-FB signaling 4 JPJDR reduced CRC liver metastasis by regulating CRC cells-derived EVs 7 JPJDR blocked the fibroblasts activation by regulating ITGBL1 expression and NF-FB signaling 7 Discussion 7 Conclusion 10 CRediT authorship contribution statement 10 Declaration of Competing Interest 11 Funding

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nonfiction