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Characterization of Enzymatic Pectin Digests by Matrix-assisted Laser Desorption/ionization Mass Spectrometry by Körner, Roman; Limberg, Gerrit; Mikkelsen, Joørn Dalgaard; Roepstorff, Peter is a Chemistry article available to read on EtoBox.

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The use of matrix-assisted laser desorption/ionization time-of-Ñight mass spectrometry for the characterization of partially methyl-esteriÐed enzymatic pectin digests is described. The sensitivities of several matrices, positive and negative ion modes and desalting techniques for these acidic oligosaccharides were compared. The most favorable results were obtained with a thin-layer preparation of a mixture of 2,4,6-trihydroxyacetophenone and nitrocellulose in the negative ion mode. Results are presented demonstrating the sensitive characterization of separated and unseparated high-ester pectin digests obtained after complete digestion using Aspergillus niger pectin lyase and the analysis of digests after chemical modiÐcation. In the case of unseparated digests, the analysis of methylation patterns is demonstrated. Oligomers with a degree of polymerization up to 40 were detected after enrichment of large oligomers by propan-2-ol precipitation.

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Author

Körner, Roman; Limberg, Gerrit; Mikkelsen, Joørn Dalgaard; Roepstorff, Peter

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley-Liss Inc; John Wiley & Sons Inc.; Wiley; Heyden And Son (ISSN 1076-5174)

Published

1998

Language

EN

Field

Chemistry (Physical Sciences)