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Rtt107/Esc4 binds silent chromatin and DNA repair proteins using different BRCT motifs by David C Zappulla; Arindel SR Maharaj; Jessica J Connelly; Rebecca A Jockusch; Rolf Sternglanz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background By screening a plasmid library for proteins that could cause silencing when targeted to the __HMR__ locus in __Saccharomyces cerevisiae__, we previously reported the identification of Rtt107/Esc4 based on its ability to establish silent chromatin. In this study we aimed to determine the mechanism of Rtt107/Esc4 targeted silencing and also learn more about its biological functions. ## Results Targeted silencing by Rtt107/Esc4 was dependent on the __SIR__ genes, which encode obligatory structural and enzymatic components of yeast silent chromatin. Based on its sequence, Rtt107/Esc4 was predicted to contain six BRCT motifs. This motif, originally identified in the human breast tumor suppressor gene __BRCA1__, is a protein interaction domain. The targeted silencing activity of Rtt107/Esc4 resided within the C-terminal two BRCT motifs, and this region of the protein bound to Sir3 in two-hybrid tests. Deletion of __RTT107/ESC4__ caused sensitivity to the DNA damaging agent MMS as well as to hydroxyurea. A two-hybrid screen showed that the N-terminal BRCT motifs of Rtt107/Esc4 bound to Slx4, a protein previously shown to be involved in DNA repair and required for viability i

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Author

David C Zappulla; Arindel SR Maharaj; Jessica J Connelly; Rebecca A Jockusch; Rolf Sternglanz

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)