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Cytokine Expression in Periapical Granulation Tissue as Assessed by Immunohistochemistry by Katie F. Walker; David F. Lappin; Keiso Takahashi; Joyce Hope; D. Gordon Macdonald; Denis F. Kinane is a Dentistry article available to read on EtoBox.
What is Cytokine Expression in Periapical Granulation Tissue as Assessed by Immunohistochemistry about?
The aims of this study were to investigate the expression of pro‐inflammatory, anti‐inflammatory and immune‐related cytokines present in periapical lesions. We investigated the expression of cytokines: namely interleukins IL‐2, IL‐4, IL‐6, IL‐10 and interferon‐γ(IFN‐γ) in formalin‐fixed, paraffin‐embedded sections of periapical granulation tissue. The study samples were biopsies from 24 patients with periapical lesions: 12 with periapical granulomas and 12 patients with radicular cysts. Immunohistochemistry was also performed on tonsillar tissue which served as a control. We utilised a set of specific monoclonal antibodies and polyclonal monospecific antibodies to detect cells that expressed the different cytokines within the tissues. We also considered the nature of the periapical immune response by investigation of the T‐helper 1 (Th‐1) and T‐helper 2 (Th‐2) lymphocyte subsets using their cytokine profile, i.e., Th‐1: IL‐2 and IFN‐γ and Th‐2: IL‐4, IL‐5 and IL‐6. Only a few cells were weakly positive for the IL‐2 protein in each of the tissue sections. Cells that expressed IL‐4 or IL‐6 were far more numerous than cells that expressed either IL‐2 or IFN‐γ. Thus, we demonstrated a
Who reads Cytokine Expression in Periapical Granulation Tissue as Assessed by Immunohistochemistry?
It is typically read by researchers, students, and practitioners in Dentistry.
- Author
- Katie F. Walker; David F. Lappin; Keiso Takahashi; Joyce Hope; D. Gordon Macdonald; Denis F. Kinane
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0909-8836)
- Published
- 2000
- Language
- EN
- Field
- Dentistry (Health Sciences)