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Can I read Characterization of a novel oncogenic K-ras mutation in colon cancer on EtoBox?
Characterization of a novel oncogenic K-ras mutation in colon cancer by Kiwamu Akagi; Ryosuke Uchibori; Kensei Yamaguchi; Keiko Kurosawa; Yoichiro Tanaka; Tomoko Kozu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Characterization of a novel oncogenic K-ras mutation in colon cancer about?
Activating mutations of RAS are frequently observed in subsets of human cancers, indicating that RAS activation is involved in tumorigenesis. Here, we identified and characterized a novel G to T transversion mutation of the K-ras gene at the third position of codon 19 (TTG) which substituted phenylalanine for leucine in 3 primary colon carcinomas. Biological and biochemical activity was examined using transformed NIH3T3 cells expressing mutant or wild-type K-ras. Transformants harboring the K-ras mutation at codon 19 showed proliferative capacity under serum-starved conditions, less contact inhibition, anchorage-independent growth, tumorigenicity in nude mice and elevation of active Ras-GTP levels. These results indicated that this novel mutation possesses high oncogenic activity.
Who reads Characterization of a novel oncogenic K-ras mutation in colon cancer?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kiwamu Akagi; Ryosuke Uchibori; Kensei Yamaguchi; Keiko Kurosawa; Yoichiro Tanaka; Tomoko Kozu
- Publisher
- Elsevier BV
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)