About this Biochemistry, Genetics and Molecular Biology article

Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design by Kimmo Rantalainen; Zachary T. Berndsen; Sasha Murrell; Liwei Cao; Oluwarotimi Omorodion; Jonathan L. Torres; Mengyu Wu; Jeffrey Umotoy; Jeffrey Copps; Pascal Poignard; Elise Landais; James C. Paulson; Ian A. Wilson; Andrew B. Ward is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Summary Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system. Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort. Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes. Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers. These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex.

Author

Kimmo Rantalainen; Zachary T. Berndsen; Sasha Murrell; Liwei Cao; Oluwarotimi Omorodion; Jonathan L. Torres; Mengyu Wu; Jeffrey Umotoy; Jeffrey Copps; Pascal Poignard; Elise Landais; James C. Paulson; Ian A. Wilson; Andrew B. Ward

Publisher

Elsevier ; Cell Press; Amsterdam: Elsevier; Research Square; Elsevier BV (ISSN 2211-1247)

Published

2018

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)