Can I read 2-Deoxy-2-Fluoro-d-Glucose as a Functional Probe for NMR: The Unique Metabolism Beyond Its 6-Phosphate on EtoBox?

2-Deoxy-2-Fluoro-d-Glucose as a Functional Probe for NMR: The Unique Metabolism Beyond Its 6-Phosphate by Y. Kanazawa; H. Yamane; S. Shinohara; S. Kuribayashi; Y. Momozono; Y. Yamato; M. Kojima; K. Masuda is a Neuroscience article available to read on EtoBox.

What is 2-Deoxy-2-Fluoro-d-Glucose as a Functional Probe for NMR: The Unique Metabolism Beyond Its 6-Phosphate about?

Abstract: Epimeric conversion of 2‐deoxy‐2‐fluoro‐d‐glucose (FDG) to its 2‐epimer 2‐deoxy‐2‐fluoro‐d‐mannose (FDM) proved by ^19^F NMR has been shown to reflect the brain activity. To examine the feasibility of FDG as a new NMR probe for in vivo functional monitoring, we studied here the fundamental NMR properties of metabolites, spectral assignments, and reliability of NMR quantification. Metabolites confirmed in brain besides FDM‐6‐phosphate were as follows: FDG‐1‐phosphate, FDG‐1,6‐bisphosphate, FDM‐1‐phosphate, FDM‐1,6‐bisphosphate, and FDG and FDM derivatives of nucleotide diphosphate. NMR quantification of these metabolites was evaluated in comparison with the method of ^18^F‐labeled FDG. In the NMR functional study using FDG, where a high dose is inevitable, the dose dependence of uptake was investigated. FDG uptake in mouse brain was shown to be in the range of interpretation using the biochemical parameters of enzymes for glucose uptake as long as a dose of <200 mg/kg was used.

Who reads 2-Deoxy-2-Fluoro-d-Glucose as a Functional Probe for NMR: The Unique Metabolism Beyond Its 6-Phosphate?

Author

Y. Kanazawa; H. Yamane; S. Shinohara; S. Kuribayashi; Y. Momozono; Y. Yamato; M. Kojima; K. Masuda

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1996

Language

EN

Field

Neuroscience (Life Sciences)