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**Analysis of nitrate regulatory protein NarL‐binding sites in the fdnG and narG operon control regions of Escherichia coli K‐12** by Andrew J. Darwin; Jing Li; Valley Stewart is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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During anaerobic growth, expression of the __fdnGHI__ and __narGHJI__ operons of __Escherichia coli__ is induced by the NarL protein in response to nitrate. The __fdnG__ operon control region contains four NarL‐binding sites (termed NarL heptamers) between positions −70 and −130. The two central NarL heptamers of __fdnG__ are arranged as an inverted repeat and are essential for regulation by NarL. We used mutational analysis of these central heptamers to investigate the precise sequence requirements for NarL‐dependent induction. Mutations were examined for their effects on NarL‐dependent expression __in vivo__. Substitutions at position 1 of either heptamer had the strongest effect whereas substitutions at position 7 had the weakest effect. For some positions, alterations in both heptamers had a stronger effect than either of the single changes. The 2 bp spacing between these NarL heptamers was also important for normal nitrate induction. The __narG__ operon control region has at least eight NarL heptamers arranged in two groups. Previous work has shown that nucleotide substitutions in two of these heptamers, centred at positions −195 and −89, severely reduce nitrate induction of _

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Author

Andrew J. Darwin; Jing Li; Valley Stewart

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)