About this Medicine article

Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap: expression of DSC2 in gastric cancer with intestinal phenotype by Katsuhiro Anami; Naohide Oue; Tsuyoshi Noguchi; Naoya Sakamoto; Kazuhiro Sentani; Tetsutaro Hayashi; Takao Hinoi; Masazumi Okajima; Jonathan M Graff; Wataru Yasui is a Medicine article available to read on EtoBox.

## Abstract Gastric cancer (GC) is one of the most common malignancies worldwide. Genes expressed only in cancer tissue, and especially on the cell membrane, will be useful molecular markers for diagnosis and may also be good therapeutic targets. To identify genes that encode transmembrane proteins present in GC, we generated __Escherichia coli__ ampicillin secretion trap (CAST) libraries from two GC cell lines and normal stomach. By sequencing 4320 colonies from CAST libraries, we identified 30 candidate genes that encode transmembrane proteins present in GC. Quantitative reverse transcription‐polymerase chain reaction analysis of these candidates revealed that __ZDHHC14, BST2, DRAM2__, and __DSC2__ were expressed much more highly in GC than in 14 kinds of normal tissues. Among these, __DSC2__ encodes desmocollin 2, which is one of three known desmocollins. Immunohistochemical analysis demonstrated that 22 (28%) of 80 GC cases were positive for desmocollin 2, and desmocollin 2 expression was observed frequently in GC with the intestinal mucin phenotype. Furthermore, desmocollin 2 expression was correlated with CDX2 expression. These results suggest that expression of desmocollin 2

Author

Katsuhiro Anami; Naohide Oue; Tsuyoshi Noguchi; Naoya Sakamoto; Kazuhiro Sentani; Tetsutaro Hayashi; Takao Hinoi; Masazumi Okajima; Jonathan M Graff; Wataru Yasui

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

2010

Language

EN

Field

Medicine (Health Sciences)