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Can I read FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells on EtoBox?
FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells by Huang, Chia-Wei; Lu, Shih-Yu; Huang, Tzu-Chieh; Huang, Bu-Miim; Sun, H. Sunny; Yang, Shang-Hsun; Chuang, Jih-Ing; Hsueh, Yuan-Yu; Wu, Yi-Ting; Wu, Chia-Ching is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells about?
**Rationale**: The formation of adipose-derived stem cells (ASCs) into spheres on a chitosan-coated microenvironment promoted ASCs differentiation into a mixed population of neural lineage-like cells (NLCs), but the underline mechanism is still unknown. Since the fibroblast growth factor 9 (FGF9) and fibroblast growth factor receptors (FGFRs) play as key regulators of neural cell fate during embryo development and stem cell differentiation, the current study aims to reveal the interplay of FGF9 and FGFRs for promoting peripheral nerve regeneration. **Methods**: Different concentration of FGF9 peptide (10, 25, 50, 100 ng/mL) were added during NLCs induction (FGF9-NLCs). The FGFR expressions and potential signaling were studied by gene and protein expressions as well as knocking down by specific FGFR siRNA or commercial inhibitors. FGF9-NLCs were fluorescent labeled and applied into a nerve conduit upon the injured sciatic nerves of experimental rats. **Results**: The FGFR2 and FGFR4 were significantly increased during NLCs induction. The FGF9 treated FGF9-NLCs spheres became smaller and changed into Schwann cells (SCs) which expressed S100β and GFAP. The specific silencing of FGFR2
Who reads FGF9 induces functional differentiation to Schwann cells from human adipose derived stem cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Huang, Chia-Wei; Lu, Shih-Yu; Huang, Tzu-Chieh; Huang, Bu-Miim; Sun, H. Sunny; Yang, Shang-Hsun; Chuang, Jih-Ing; Hsueh, Yuan-Yu; Wu, Yi-Ting; Wu, Chia-Ching
- Publisher
- Ivyspring International Publisher; Wyoming N.S.W.: Ivyspring International Publisher, 2011- (ISSN 1838-7640)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)