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Molecular Phylogenetics Reveals the Diversity of Antagonistic Fungal Endophytes Inhabiting Medicinal Plants in Nigeria by Chijioke E. Ezeobiora; Nwamaka H. Igbokwe; Dina H. Amin; Udoma E. Mendie is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Molecular Phylogenetics Reveals the Diversity of Antagonistic Fungal Endophytes Inhabiting Medicinal Plants in Nigeria about?

spp. Using preliminary antimicrobial tests, 17 of the isolates demonstrated antimicrobial activity against at least one of the eight tested pathogens. The phylogenetic analysis of these isolates revealed that 11 of them are divergent strains, emerging as a monophyletic clade. These endophytes are reported for the first time in the Nigerian medicinal plants investigated. In conclusion, endophytic fungi associated with ethnomedicinal plants in Nigeria could be a source of new endophytes, which could lead to the development of novel antimicrobials. Keywords Endophytic fungi • Medicinal plants • ITS sequencing • Phylogenetic analysis • Antimicrobial activity Abbreviations MEGA Molecular evolutionary genetic analysis MUSCLE Multiple sequence alignment by log expectation DNA Deoxyribonucleic acid PCR Polymerase chain reaction CF Colonization frequency ITS Internal transcribed spacer PDA Potato dextrose agar NA Nutrient agar MHA Mueller Hinton agar IZ Inhibition zone IZD Inhibition zone diameter BLAST Basic local alignment search tool LPCB Lactophenol cotton blue rDNA Ribosomal deoxyribonucleic acid BlastN Nucleotide basic local alignment search toolAbstract Antimicrobial resistance is a

Who reads Molecular Phylogenetics Reveals the Diversity of Antagonistic Fungal Endophytes Inhabiting Medicinal Plants in Nigeria?

Author

Chijioke E. Ezeobiora; Nwamaka H. Igbokwe; Dina H. Amin; Udoma E. Mendie

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)