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Active MMP-2 effectively identifies the presence of colorectal cancer by Mary Jo Murnane; Jinguo Cai; Sania Shuja; David McAneny; Veronica Klepeis; John B. Willett is a Medicine article available to read on EtoBox.

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## Abstract Fully active MMP‐2 is expressed at such low levels in human tissues that studies often fail to confirm its value as a cancer marker despite strong associations with malignancy. Our study utilized careful extraction, accurate activity measurements, standardization to purified controls and a new statistical metric to determine whether active MMP‐2 is an effective indicator of colorectal cancer compared to pro‐MMP‐2 or pro‐MMP‐9. MMP‐2 and MMP‐9 activities were analyzed in matched normal and cancer samples from 269 patients by gelatin zymography, computer‐assisted image analysis, serial dilutions of strong samples and standardization to controls. An index of effect size was designed for comparative evaluation of active MMP‐2, pro‐MMP‐2 and pro‐MMP‐9 activities. For each gelatinase, mean activity and protein levels/mg soluble protein in normal mucosa and colorectal cancer were calculated for the first time with respect to commercial standards. Active MMP‐2 activity, detected in 99% of colorectal cancers, was higher in 95% of cancers (on average 10‐fold) than in normal mucosa. Levels of pro‐MMP‐2 and pro‐MMP‐9, but not active MMP‐9, activities were also significantly higher

Who reads Active MMP-2 effectively identifies the presence of colorectal cancer?

Author

Mary Jo Murnane; Jinguo Cai; Sania Shuja; David McAneny; Veronica Klepeis; John B. Willett

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2009

Language

EN

Field

Medicine (Health Sciences)