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Can I read METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer on EtoBox?
METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer by Kangyue Sun; Lu Chen; Yiwen Li; Bing Huang; Qun Yan; Changjie Wu; Qiuhua Lai; Yuxin Fang; Jianqun Cai; Yongfeng Liu; Junsheng Chen; Xinke Wang; Yuxuan Zhu; Shuyu Dong; Jieyu Tan; Aimin Li; Side Liu; Yue Zhang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer about?
## Abstract miR-17-5p has been found to be involved in the proliferation and metastasis of colorectal cancer (CRC), and N^6^-methyladenosine (m^6^A) modification is the most common RNA modification in eukaryotes. However, whether miR-17-5p contributes to chemotherapy sensitivity in CRC via m^6^A modification is unclear. In this study, we found that overexpression of miR-17-5p led to less apoptosis and lower drug sensitivity in vitro and in vivo under the 5-fluorouracil (5-FU) treatment, which indicated miR-17-5p led to 5-FU chemotherapy resistance. Bioinformatic analysis suggested that miR-17-5p-mediated chemoresistance was associated with mitochondrial homeostasis. miR-17-5p directly bound to the 3’ untranslated region of Mitofusin 2 (MFN2), leading to decreased mitochondrial fusion and enhanced mitochondrial fission and mitophagy. Meanwhile, methyltransferase-like protein 14 (METTL14) was downregulated in CRC, resulting in lower m^6^A level. Moreover, the low level of METTL14 promoted the expression of pri-miR-17 and miR-17-5p. Further experiments suggested that m^6^A mRNA methylation initiated by METTL14 inhibits pri-miR-17 mRNA decay via reducing the recognition of YTHDC2 to th
Who reads METTL14-dependent maturation of pri-miR-17 regulates mitochondrial homeostasis and induces chemoresistance in colorectal cancer?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kangyue Sun; Lu Chen; Yiwen Li; Bing Huang; Qun Yan; Changjie Wu; Qiuhua Lai; Yuxin Fang; Jianqun Cai; Yongfeng Liu; Junsheng Chen; Xinke Wang; Yuxuan Zhu; Shuyu Dong; Jieyu Tan; Aimin Li; Side Liu; Yue Zhang
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)