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Can I read Resolution of a Coeluting Chromatographic Pair Using Kalman Filtering on EtoBox?
Resolution of a Coeluting Chromatographic Pair Using Kalman Filtering by Todd Barker; Steven D. Brown is a Chemistry article available to read on EtoBox.
What is Resolution of a Coeluting Chromatographic Pair Using Kalman Filtering about?
A new approach is reported for enhancing resolution in chromatographic separations monitored by diode array spectroscopy. This approach uses a digital filter, known as the Kalman filter, to resolve coeluting chromatographic components by fitting the time-dependent spectral responses observed at the detector to the spectra of pure component models. The estimates of concentration as a function of time permit the creation of elution profiles for each chromatographic component included in the model. The method is tested on synthetic three-dimensional chromatograms generated from Gaussian elution profiles, and it is demonstrated that, with adequate spectral models, the overlapped chromatographic components can be resolved. Arbitrarily poor chromatographic resolution, jitter in peak location, and high detector noise do not interfere. The method was applied to the separation of overlapped chromatographic peaks from dopamine and tyrosine, and it was demonstrated that accurate quantitation was possible, even when chromatographic resolution was less than ideal. The method fails when spectral responses occur that are not included in the filter model.
Who reads Resolution of a Coeluting Chromatographic Pair Using Kalman Filtering?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Todd Barker; Steven D. Brown
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1989
- Language
- EN
- Field
- Chemistry (Physical Sciences)