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The PdeWRKY65-UGT75L28 gene module negatively regulates lignin biosynthesis in poplar petioles by Tashbek Nvsvrot; Xiaoqing Yang; Yan Zhang; Liyu Huang; Guanghua Cai; Yiwei Ding; Wenyu Ren; Nian Wang is a Engineering article available to read on EtoBox.

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Lignin is made up of three main p-hydroxycinamyl alcohol precursors or monolignols. Lignin biosynthesis is not only important for plant growth, but also the key factor for human use of plants as biofuels. In past decades, there were numerous studies have focused on lignification in plant stems; however, little has been uncovered about lignification in petioles. In this study, a gene encoding a glycosyltransferase was identified in poplar and named UGT75L28. A relatively higher expression of UGT75L28 could be observed in petioles. Transgenic lines overexpressing UGT75L28 showed reduced lignification in petioles, while reduced expression (RE) of this gene displayed opposite phenotype. The protein of UGT75L28 fused with six His tags was expressed in E. coli and purified. In vitro enzyme reactions were conducted for a number of substrates for this fused protein. The results revealed that UGT75L28 could only glycosylate coniferyl aldehyde, which is a substrate in lignin biosynthesis in plants. The identification of the upstream regulator of UGT75L28 found that the transcription factor PdeWRKY65 could bind to the promoter of UGT75L28 and activate its expression both in vivo and in vitro

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Author

Tashbek Nvsvrot; Xiaoqing Yang; Yan Zhang; Liyu Huang; Guanghua Cai; Yiwei Ding; Wenyu Ren; Nian Wang

Publisher

Elsevier BV

Published

2023

Language

EN

Field

Engineering (Physical Sciences)