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Genetic evidence for a functional interaction between Saccharomyces cerevisiae CDC24 and CDC42 by Michael Ziman; Dr Douglas I. Johnson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Cdc24p and Cdc42p are involved in the control of cell polarity during the __Saccharomyces cerevisiae__ cell cycle. Cdc42p is a member of the Ras superfamily of GTPases and Cdc24p displays limited amino‐acid sequence similarity with the Dbl proto‐oncoprotein, which acts to stimulate guanine‐nucleotide exchange on human Cdc42p. We have performed several genetic experiments to test whether Cdc24p and Cdc42p interact within the cell. First, overexpression of Cdc24p suppressed the dominant‐negative __cdc42__^D118A^ allele. Second, overexpression of wild‐type __CDC24__ and __CDC42__ genes together was a lethal event resulting in a morphological phenotype of large, round, unbudded cells, indicating a loss of cell polarity. Third, a __cdc24__^ts^ __cdc42__^ts^ double mutant exhibited a synthetic‐lethal phenotype at the semi‐permissive temperature of 30°C. These data suggest that Cdc24p and Cdc42p interact within the cell and that Cdc24p may be involved in the regulation of Cdc42p activity.

Who reads Genetic evidence for a functional interaction between Saccharomyces cerevisiae CDC24 and CDC42?

Author

Michael Ziman; Dr Douglas I. Johnson

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)