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The bifactorial endosperm box of γ-zein gene: characterisation and function of the Pb3 and GZMcis-acting elements by Pau Marzábal; Peter K. Busk; M. Dolors Ludevid; Margarita Torrent is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Summary The proximal region of the γ‐zein promoter (γ __Z__ ) has a functional bifactorial prolamin box element containing two __cis__ ‐acting elements, a prolamin‐box motif (Pb3) and a GCN4‐like motif (GZM). By particle bombardment of maize endosperms with 5′ deletions and internal deletions of γ __Z__ fused to the GUS gene, we have shown that a 135 bp region containing the bifactorial element is involved in the transcriptional activation of the γ __Z__ promoter. However, the 135 bp region was unable to activate the γ __Z__ promoter in the absence of a 84 bp downstream sequence. Using __in vivo__ footprinting and gel mobility shift assays with 15 DAP endosperm nuclear extracts, we have demonstrated the presence of __trans__ ‐acting factors that interact with Pb3 and GZM target sites. Base‐substitution mutations within Pb3 and GZM decreased transcription activity of the γ __Z__ promoter suggesting a co‐ordinated function between the two __cis__ ‐acting elements. Two additional __cis__ ‐motifs upstream of the bifactorial prolamin element have been identified: a motif with high homology to the AACA elements of rice glutelin genes and an AZM motif containing an ACGT core which bind

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Author

Pau Marzábal; Peter K. Busk; M. Dolors Ludevid; Margarita Torrent

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1998

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)