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Can I read Expression of a dominant negative form of cpSRP54 inhibits chloroplast biogenesis in Arabidopsis on EtoBox?
Expression of a dominant negative form of cpSRP54 inhibits chloroplast biogenesis in Arabidopsis by Marsha L. Pilgrim; Klaas-Jan Va; Wijk; Devin H. Parry; Donna A. C. Sy; Neil E. Hoffman is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Expression of a dominant negative form of cpSRP54 inhibits chloroplast biogenesis in Arabidopsis about?
The chloroplast homolog of the 54 kDa subunit of signal recognition particle is required for the in vitro targeting of chlorophyll a/b binding proteins (LHCP) to the thylakoid membrane. To explore the function of cpSRP54 in vivo, plants that are mutated in cpSRP54 function were generated. Dominant negative forms of cpSRP54 altered in single amino acids within the conserved guanine nucleotide binding domain were expressed in Arabidopsis. Transformed plants contained less than 30% of the wild-type level of cpSRP54 protein. As a consequence of the reduced cpSRP54 protein content, the first emerging leaves were yellow and contained immature chloroplasts. Although the chlorophyll (chl) content of the leaves was reduced by 75%, the chl a/b ratio was unaffected, indicating a role of cpSRP54 in the biogenesis of proteins besides LHCP. Many chloroplast proteins were less abundant in the first emerging leaves, including non-pigmented proteins, thylakoid proteins known to be targeted by alternative pathways, and soluble proteins. These observations indicate that the cpSRP54 mutation also has a pleiotropic effect on chloroplast biogenesis. Whereas the level of cpSRP54 remained low as the plant
Who reads Expression of a dominant negative form of cpSRP54 inhibits chloroplast biogenesis in Arabidopsis?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Marsha L. Pilgrim; Klaas-Jan Va; Wijk; Devin H. Parry; Donna A. C. Sy; Neil E. Hoffman
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)
- Published
- 1998
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)