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Simultaneous Quantification of Cefotaxime, Desacetylcefotaxime, Ofloxacine and Ciprofloxacine in Ocular Aqueous Humor and in Plasma by High-performance Liquid Chromatography by Hans-Joachim Kraemer; Ralf Gehrke; Antje Breithaupt; Henning Breithaupt is a Chemistry article available to read on EtoBox.

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Cefotaxime, given intravenously, is currently used as a broad-spectrum antibiotic for prophylaxis of intra- and postoperative infections in ocular lens surgery. A proposed therapeutic and economic alternative is the use of orally active fluoroquinolone ofloxacine as prophylactic agent. A HPLC method was developed for determination of both antibiotics in ocular aqueous humor and plasma in order to optimize dosage for safe surpassing minimal inhibitory concentration in the humor compartment. For plasma determinations a solid-phase extraction procedure was used with ciprofloxacine as internal standard. Detection limits for direct HPLC-analysis of ocular aqueous humor was 0.08 microg/ml for all compounds, whereas in plasma 0.31 microg/ml could be determined after solid-phase extraction.

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Author

Hans-Joachim Kraemer; Ralf Gehrke; Antje Breithaupt; Henning Breithaupt

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1997

Language

EN

Field

Chemistry (Physical Sciences)