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Can I read Preparation of a verifiable peptide-protein immunogen: Direction-controlled controlled conjugation of a synthetic fragment of the monitor peptide with myoglobin and application for sequence analysis on EtoBox?
Preparation of a verifiable peptide-protein immunogen: Direction-controlled controlled conjugation of a synthetic fragment of the monitor peptide with myoglobin and application for sequence analysis by Kazuo Iwai; Shin-Ichi Fukuoka; Tohru Fushiki; Keishi Kido; Yoji Sengoku; Toshihiko Semba is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Preparation of a verifiable peptide-protein immunogen: Direction-controlled controlled conjugation of a synthetic fragment of the monitor peptide with myoglobin and application for sequence analysis about?
A useful method for preparing a synthetic peptide-carrying protein for specific antibody production was established. The monitor peptide is a trypsin-sensitive cholecystokinin-releasing peptide purified from rat pancreatic juice on the basis of its stimulatory activity toward pancreatic enzyme secretion. The NH2-terminus fragment of the monitor peptide (residues 1-14) was synthesized by a solid phase method. Cysteine at the COOH terminus of the fragment was conjugated with amino groups of myoglobin using a hetero-bifunctional reagent. Sequence analysis of the fragment-myoglobin conjugate indicated that the peptide/myoglobin conjugation ratio was about 1/1 (mol/mol). Antiserum against the conjugate from a rabbit effectively abolished the stimulatory activity of the monitor peptide in the rat small intestine.
Who reads Preparation of a verifiable peptide-protein immunogen: Direction-controlled controlled conjugation of a synthetic fragment of the monitor peptide with myoglobin and application for sequence analysis?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kazuo Iwai; Shin-Ichi Fukuoka; Tohru Fushiki; Keishi Kido; Yoji Sengoku; Toshihiko Semba
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1988
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)