Can I read The involvement of nitric oxide in the anti-Candida albicans activity of rat neutrophils on EtoBox?

The involvement of nitric oxide in the anti-Candida albicans activity of rat neutrophils by I. M. FIERRO; C. BARJA-FIDALGO; F. Q. CUNHA; S. H. FERREIRA is a Immunology and Microbiology article available to read on EtoBox.

What is The involvement of nitric oxide in the anti-Candida albicans activity of rat neutrophils about?

Rat peritoneal neutrophils (PMN) spontaneously release nitric oxide (NO) when incubated __in vitro__. Addition of the NO synthase inhibitor L‐monomethylarginine (L‐NMMA) to the PMN reduces NO production and impairs the killing of the yeast __Candida albicans__, both effects being reversed by L‐arginine. These data strongly suggest that oxidative metabolism of L‐arginine by PMN is involved in the candidacidal activity of these cells. Rat blood PMN, which do not produce significant amounts of NO, exhibit a reduced killing capacity compared with peritoneal cells, except when they are obtained from lipopolysaccharide (LPS)‐treated rats. In this case they produce measurable amounts of nitrite and express high fungicidal activity __in vitro__. Confirming the candidacidal activity of NO, the exposure of the __C. albicans__ cultures to different concentrations of NO donors leads to a reduction in their survival. The candidacidal activity related to the NO pathway in rat PMN is phagocytosis dependent, since the activity can be inhibited by cytochalasin B. However, the oxidative products of oxygen released by rat PMN do not seem to be involved in their candidacidal activity, as incubation of

Who reads The involvement of nitric oxide in the anti-Candida albicans activity of rat neutrophils?

Author

I. M. FIERRO; C. BARJA-FIDALGO; F. Q. CUNHA; S. H. FERREIRA

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0019-2805)

Published

1996

Language

EN

Field

Immunology and Microbiology (Life Sciences)