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This paper studies the relationship between DNA extension, torque, and supercoiling under tension. It finds that the maximum in the extension curve during supercoiling is not indicative of a phase transition from B-DNA to scP-DNA, as previously thought. Using direct torque measurements, it identifies the phase transition by a torque plateau. A theory incorporating twist-stretch coupling and bending fluctuations can explain the extension curve shape. This clarifies the signatures of twist-stretch coupling ve

Author

Lokosoo

Language

EN