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Metal Inhibition of Calmodulin Activity in Monkey Brain by P. J. S. Vig; R. Nath; D. Desaiah is a Environmental Science article available to read on EtoBox.

What is Metal Inhibition of Calmodulin Activity in Monkey Brain about?

Studies were conducted to investigate the effect of vanadium (V5+), cadmium (CdZ+), mercury (Hg2+ ), aluminium (A13+), lead (Pb2') and manganese (Mn") ions on calmodulin (CaMkrelated Ca2'-ATPase activity in rhesus monkey (Macaca mulatta) brain (RMB). The cerebral cortex of RMB was dissected and homogenized in 10% sucrose buffer, and synaptic plasma membranes (SPM) were prepared. Ca2+-ATPase activity was determined by the inorganic phosphate method. Different concentrations of metal ions were incubated with RMB fractions containing endogenous CaM and RMB fractions without CaM. The results show that no metal ion inhibited the basal enzyme but that they showed a profound inhibition of total Ca2' -ATPase containing CaM. The order of potency of metals was Hg>Cd>Pb>Mn>AI>V. Exogenous addition of CaM restored the metal-inhibited enzyme activity to near the normal level. The basal enzyme, when reconstituted with CaM, showed sensitivity to metal ions. These data, presented for the first time in non-human primate brain, indicate that the metal ions inhibit CaM activity in RMB.

Who reads Metal Inhibition of Calmodulin Activity in Monkey Brain?

Author

P. J. S. Vig; R. Nath; D. Desaiah

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0260-437X)

Published

1989

Language

EN

Field

Environmental Science (Physical Sciences)